Isolation of Trypsin from Serum of pancreatic Cancer Patients and determination some biochemical parameters

The research included isolation and purification of Trypsin from serum patient with pancreatic cancer, estimation its molecular weight by using the two techniques gel filtration (using sephadex G-50) electrophoresis SDS). Therefore; it was noted that proteinous peak isolated ammonium sulfate solution is saturated (65%) had apparent (27000) Dalton. study (40) patients pancreas cancer their ages ranged between (30-70) years who attended Al-Jumhuri Atomic Medicine Hospitals in Mosul; laboratory examination levels of: Trypsin, Gamma Glutamyl transferase, Amylase, Paraoxonase ,Glutathione, Sialic acid Ceruloplasmin. Results showed level Amylase decreased significantly comparison control group, but paraoxonase, Glutathione, Ceruloplasmin increased group.